[Comparison of candidal and bacterial adherence to denture base acrylic resins]

Futura Gen and GC Reline Hard denture chairside reline materials have recently been introduced to the dental market. Although yet to be scientifically proven, these products are claimed to have optimal characteristics. This study aimed to compare the adherence of Candida strains and bacteria to denture base acrylic resins. In this in-vitro experimental study, 24 discs were fabricated of Futura Gen, GC Reline Hard and Meliodent reline acrylic resins. Specimens were inoculated with three Candida strains and Streptococcus mutans at three time points and after staining, their optical absorbance [microbial adhesion and biofilm formation] was calculated using spectrophotometry. Two-way ANOVA and Tukey's HSD test were applied for statistical analysis. Absorbance [optical density]of C. albicans strains on Futura Gen, GC Reline and Meliodent hard reline acrylic resins was 0.022, 0.011 and 0.028 at one hour, 0.057, 0.022 and 0.062 at 24 hours and 0.101, 0.051 and 0.11 at one week, respectively. Absorbance of C. glabrata on the mentioned resins was 0.012, 0.008 and 0.016 at one hour, 0.039, 0.029 and 0.044 at 24 hours and 0.075, 0.068 and 0.081 at one week, respectively. Absorbance of C. dubliniensis on these resins was 0.026, 0.035 and 0.027 at one hour, 0.064, 0.066 and 0.067 at 24 hours and 0.11, 0.12 and 0.13 at one week, respectively. Absorbance of standard strain of S. mutans was 0.027, 0.014 and 0.035 at one hour, 0.064, 0.026 and 0.064 at 24 hours and 0.11, 0.05 and 0.11 at one week, respectively. Candidal and bacterial adhesion to denture base acrylic resins was not significantly different at the understudy time points [P>0.05]. Except for C. dubliniensis, the lowest absorbance belonged to Meliodent. However, absorbance increased over time. Optical absorbance of S. mutans was lower in Meliodent. Among yeast strains, the highest absorbance belonged to C. dubliniensis

In vitro study of the effect of clotrimazole incorporation into silicone soft liner on fungal colonization

Candidal colonization on soft liners is important in the development of pathogenesis, such as denture stomatitis. It has heen reported that combining anti-fungal agents into soft liners might be used in treatment and prevention of denture stomatitis. The aim of this in-vitro study was to determine whether incorporating Clotrimazole [C] into the silicone soft liner [S], would inhibit the growth of C. albicans when the specimens are stored in distilled water and washed daily with wet cotton. Experimental specimen disks incorporated with Clotrimazole [Sc[1], Sc[30], Sc[60] and without Clotrimazole [S[1], S[30], S[60]] into the soft liner [no = 8] were fabricated aseptically against the polyester film to produce a smooth surface. The treated and control disks were stored in distilled water for 1, 30 and 60 days and washed daily with wet cotton. For fungal growth assessment, they were inoculated with C .albicans suspension. The disks were rinsed and sonicated in sterile water to remove surface organisms. The attached yeast was measured by inoculation of the yeast suspension on Sabouraud's agar. The data were compared using two-way ANOVA. The mean colony forming units [CPU] per mm[2] for the specimens without Clotrimazole after water storage and washing with wet cotton for 1, 30 and 60 days was 6.5X10[6], 5.8X10[6] and 6.1X10[6], respectively. CPU for specimens with Clotrimazole decreased significantly to 2.6X10[6] and 3.9X10[6] and 4.6X10[6] after 1, 30, and 60 days, respectively. In comparison to those of the control disks, Clotrimazole in treated disks was effective in inhibiting C.albicans growth significantly following storage in water for 2 months [P < 0.05]. The addition of Clotrimazole significantly reduced C.albicans growth to the surface of the silicone soft liner. The samples continued to inhibit the fungal growth when they were washed daily with wet cotton for 2 months

[ effects of antifungal azoles on inflammatory cytokine production in human keratinocytes]

Azoles drugs are being used successfully in treatment of fungal infections. Recently, immunosuppressive effects of some of these agents have been reported. Keratinocytes as the major cells of the skin, have an important role in innate immunity against pathogenic agents. Considering the scanty of information about the effects of azoles on immune responces, this study was conducted to assess the expression and secretion of inflammatory cytokines in keratinocytes following treatment with azole drugs. This is an exprimental study conducted in molecular biology division in Tehran University of Medical Sciences and Immunodermatology Department in Vienna Medical University. Primery keratinocytes were cultured and treated with different concentrations of fluconazole, itraconazole, ketoconazole and griseofulvin. Secreted IL1, IL6 and TNF-alpha by keratinocytes in culture supernatant were measured by quantitative enzyme immunoassay technique. Moreover, expression of the genes encoding IL1 and IL8 was evaluated by Real Time-PCR. Treatment of keratinocytes with different concentrations of fluconazole and low concentration of ketoconazole resulted in decrease in IL1 secretion, but itraconazole and griseofulvin did not show such an effect at the same concentrations. In addition, none of the examined drugs had an effect on secretion level of IL6 and TNF-alpha. Quantitative analysis of IL1 and IL8 encoding genes revealed that transcription on these genes might be suppressed following treatment with fluconazole or ketoconazole. Fluconazole and ketoconazole might modulate the expression and secretion of IL1 and IL8 and affect the direction of immune responses induced by keratinocytes

case of actinomycotic mycetoma in hand treated successfully with co-trimoxazole and streptomycin

Mycetoma is a chronic infection involving cutaneous and subcutaneous tissues. We describe a 33-year old man who presented subcutaneous nodules with draining sinuses that extrude masses of the infecting organism. The etiologic agent of disease was identified as Nocardia asteroids by direct examination, culture of exudates and physiological tests. The lesions were completely eradicated by using combination of streptomycin and co-trimoxazole